| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Temni, Matthew (9)
|
MRFREE |
45.98 |
43.22 |
-2.76 |
| 1 |
Haridasan, Nikolai (9)
|
MRYFD |
44.08 |
44.30 |
0.22 |
| 2 |
Gutin, Noah (9)
|
MRMPNY |
43.70 |
44.43 |
0.73 |
| 2 |
Doyle, Charles (9)
|
MRCAS |
46.95 |
49.06 |
2.11 |
| 3 |
Chao, Jacob M (9)
|
MRCAS |
46.46 |
44.79 |
-1.67 |
| 3 |
Gelvez-Cisneros, Alexander (9)
|
MRLGAC |
48.32 |
49.23 |
0.91 |
| 4 |
Ioselevich, Sam (8)
|
MRTRI$ |
46.28 |
44.98 |
-1.30 |
| 4 |
Deguzman, Michael (9)
|
MRHES |
48.40 |
50.14 |
1.74 |
| 5 |
Nwosu, Tobenna D (9)
|
MRCIY |
45.16 |
45.20 |
0.04 |
| 6 |
Sorenson, Paulo S (9)
|
MRWEST |
47.11 |
45.37 |
-1.74 |
| 7 |
Attenborough, Michael (9)
|
MRFREE |
45.78 |
46.33 |
0.55 |
| 8 |
Neal, Delano K (9)
|
MRLGAC |
46.94 |
46.68 |
-0.26 |
| 9 |
Kolodziej, Marcel (9)
|
MRTRI$ |
48.30 |
46.79 |
-1.51 |
| 10 |
Bowen, Julian P (9)
|
MRLGAC |
46.62 |
47.54 |
0.92 |
| 11 |
Pan, John (8)
|
MRQNS |
44.57 |
47.72 |
3.15 |
| 12 |
Comia, Jacob (9)
|
MRFREE |
46.96 |
47.94 |
0.98 |
| 13 |
Chan, Preston (9)
|
MRLIE |
47.05 |
48.09 |
1.04 |
| 14 |
Bisono, Aaron L (9)
|
MRFLY |
47.93 |
48.29 |
0.36 |
|
Hill, Kai B (9)
|
MRBAD |
47.54 |
DQ |
- |